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Effect of MSCs on cytokines. ELISA analysis of TNF‐α, IL‐6, IL‐17, and TGF‐β expression in serum after MSC intervention (control n = 4, CIA n = 9, and MSC n = 8). Data are presented as mean ± SD and were analyzed via one‐way ANOVA followed by Tukey’s post hoc tests. # p < 0.05 Control vs. CIA, ## p < 0.01 control vs. CIA, and ∗ p < 0.05 CIA vs. MSC.

Journal: Journal of Immunology Research

Article Title: Metabolic Reprogramming of T Cells by MSCs Rebalances Th17/Treg Axis to Attenuate Collagen‐Induced Arthritis

doi: 10.1155/jimr/1862250

Figure Lengend Snippet: Effect of MSCs on cytokines. ELISA analysis of TNF‐α, IL‐6, IL‐17, and TGF‐β expression in serum after MSC intervention (control n = 4, CIA n = 9, and MSC n = 8). Data are presented as mean ± SD and were analyzed via one‐way ANOVA followed by Tukey’s post hoc tests. # p < 0.05 Control vs. CIA, ## p < 0.01 control vs. CIA, and ∗ p < 0.05 CIA vs. MSC.

Article Snippet: Cytokines levels (TNF‐α, IL‐6, IL‐17, and TGF‐β) were quantified using ELISA kits (ABclonal, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control

SCLC-N-driven macrophage polarization is IGFBP2-dependent. ( A ) Bar plot showing the correlation between IGFBP2 expression and immune cell infiltration in SCLC, based on CIBERSORT analysis of the GSE60052 dataset. ( B - C ) Protein and transcript levels of CD163 and IGFBP2 in tumor tissues were examined by mIHC ( B ) and RT-qPCR ( C ), respectively. ( D - F ) M0-THP-1 cells were treated with rhIGFBP2, and the mRNA and protein levels of M2-TAM markers (CD163 and HLA) were assessed by RT-qPCR and Western blotting ( D - E ), while cytokine levels (IL-10 and IL-12) in the culture supernatants were measured by ELISA ( F ). ( G - L ) M0-THP-1 cells were co-cultured with the indicated SCLC cells with IGFBP2 knockdown (sgIGFBP2). The mRNA and protein levels of M2-TAM markers (CD163 and HLA) were assessed by RT-qPCR and Western blot ( G - J ), while cytokine levels (IL-10 and IL-12) in the culture supernatants were measured by ELISA ( K - L )

Journal: Journal of Translational Medicine

Article Title: IGFBP2 promotes immunosuppression by regulating macrophage PD-L1 expression in NEUROD1-high small cell lung cancer

doi: 10.1186/s12967-026-08112-2

Figure Lengend Snippet: SCLC-N-driven macrophage polarization is IGFBP2-dependent. ( A ) Bar plot showing the correlation between IGFBP2 expression and immune cell infiltration in SCLC, based on CIBERSORT analysis of the GSE60052 dataset. ( B - C ) Protein and transcript levels of CD163 and IGFBP2 in tumor tissues were examined by mIHC ( B ) and RT-qPCR ( C ), respectively. ( D - F ) M0-THP-1 cells were treated with rhIGFBP2, and the mRNA and protein levels of M2-TAM markers (CD163 and HLA) were assessed by RT-qPCR and Western blotting ( D - E ), while cytokine levels (IL-10 and IL-12) in the culture supernatants were measured by ELISA ( F ). ( G - L ) M0-THP-1 cells were co-cultured with the indicated SCLC cells with IGFBP2 knockdown (sgIGFBP2). The mRNA and protein levels of M2-TAM markers (CD163 and HLA) were assessed by RT-qPCR and Western blot ( G - J ), while cytokine levels (IL-10 and IL-12) in the culture supernatants were measured by ELISA ( K - L )

Article Snippet: Cytokine levels (TNF-α, IFN-γ, IL-10, and IL-12) were quantified by ELISA using the following kits (Human TNF-α, IFN-γ, IL-10, and IL-12 ELISA Kits; HUABIO, China; Cat#EH0002, EH0039, EH0009, MQ00501S).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture, Knockdown